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Optimising in-cell NMR acquisition for nucleic acids
Optimising in-cell NMR acquisition for nucleic acids
Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and...
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Side chain isotope labelling is a powerful tool to study protein structure and interactions by NMR spectroscopy. 1H,13C la...
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
The dynamics of the backbone and side-chains of protein are routinely studied by interpreting experimentally determined 15...
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
The recent application of 19F NMR in the study of biomolecular structure and dynamics has made it a potentially attractive...
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight
A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium f...
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium (2H) spin relaxation of 13CH2D methyl groups has been widely applied to investigate picosecond-to-nanosecond con...
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa wherea...
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Solution NMR spectroscopy is a particularly powerful technique for characterizing the functional dynamics of biomolecules,...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, mater...
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
A transverse relaxation optimized spectroscopy (TROSY) approach is described for the optimal detection of NH2 groups in as...
Fluorine labelling for in situ 19F NMR in oriented systems
Fluorine labelling for in situ 19F NMR in oriented systems
The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19F to determine the orientat...
A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. T...
Short and long range 2D 15N–15N NMR correlations among peptide groups by novel solid state dipolar mixing schemes
Short and long range 2D 15N–15N NMR correlations among peptide groups by novel solid state dipolar mixing schemes
A recently developed homonuclear dipolar recoupling scheme, Adiabatic Linearly FREquency Swept reCOupling (AL FRESCO), was...
Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale
Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale
Although NMR spectroscopy is routinely used to study the conformational dynamics of biomolecules, robust analyses of the d...
The NMR signature of maltose-based glycation in full-length proteins
The NMR signature of maltose-based glycation in full-length proteins
Reducing sugars can spontaneously react with free amines in protein side chains leading to posttranslational modifications...
Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns
Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns
We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chai...
DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu
DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu
With the sensitivity enhancements conferred by dynamic nuclear polarization (DNP), magic angle spinning (MAS) solid state ...
Prediction of order parameters based on protein NMR structure ensemble and machine learning
Prediction of order parameters based on protein NMR structure ensemble and machine learning
The fast motions of proteins at the picosecond to nanosecond timescale, known as fast dynamics, are closely related to pro...
Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab
Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab
Monoclonal antibodies (mAbs) are biotherapeutics that have achieved outstanding success in treating many life-threatening ...
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensi...
19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor
19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor
For the A2A adenosine receptor (A2AAR), a class A G-protein-coupled receptor (GPCR), reconstituted in n-dodecyl-β-D-m...
Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae
Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae
Despite the prevalence and importance of glycoproteins in human biology, methods for isotope labeling suffer significant l...
5D solid-state NMR spectroscopy for facilitated resonance assignment
5D solid-state NMR spectroscopy for facilitated resonance assignment
1H-detected solid-state NMR spectroscopy has been becoming increasingly popular for the characterization of protein struct...
Efficient determination of the accessible conformation space of multi-domain complexes based on EPR PELDOR data
Efficient determination of the accessible conformation space of multi-domain complexes based on EPR PELDOR data
Many proteins can adopt multiple conformations which are important for their function. This is also true for proteins and ...
Paramagnetic NMR to study iron sulfur proteins: 13C detected experiments illuminate the vicinity of the metal center
Paramagnetic NMR to study iron sulfur proteins: 13C detected experiments illuminate the vicinity of the metal center
The robustness of NMR coherence transfer in proximity of a paramagnetic center depends on the relaxation properties of the...
Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics
Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics
In this study, we present the synthesis and incorporation of a metabolic isoleucine precursor compound for selective methy...
Breaking boundaries: TINTO in POKY for computer vision-based NMR walking strategies
Breaking boundaries: TINTO in POKY for computer vision-based NMR walking strategies
Nuclear magnetic resonance is a crucial technique for studying biological complexes, as it provides precise structural and...
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by prolonging the measurement time...
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
In protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisit...
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