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Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins
Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins
Unbiased forward genetic screens have been extensively employed in biological research to elucidate functional genomics. I...
Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells
Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells
Durable and functional regeneration of the airway epithelium in vivo with transplanted stem cells has the potential to rec...
Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes
Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes
Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-di...
Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments
Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments
We are thankful for the support of the Ministry of Science, Research and the Arts of Baden-Württemberg (MWK Baden-Württemb...
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Superstructures with architectural complexity and unique functionalities are promising for a variety of practical applicat...
High-throughput glycosaminoglycan extraction and UHPLC-MS/MS quantification in human biofluids
High-throughput glycosaminoglycan extraction and UHPLC-MS/MS quantification in human biofluids
Glycosaminoglycans (GAGs) are linear, unbranched heteropolysaccharides whose structural complexity determines their functi...
RNA sample optimization for cryo-EM analysis
RNA sample optimization for cryo-EM analysis
RNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily de...
Host circuit engagement of human cortical organoids transplanted in rodents
Host circuit engagement of human cortical organoids transplanted in rodents
Human neural organoids represent promising models for studying neural function; however, organoids grown in vitro lack cer...
High-resolution topographic surveying and change detection with the iPhone LiDAR
High-resolution topographic surveying and change detection with the iPhone LiDAR
This paper introduces a comprehensive protocol leveraging open-access techniques to create small- to medium-scale 3D repre...
A network control theory pipeline for studying the dynamics of the structural connectome
A network control theory pipeline for studying the dynamics of the structural connectome
Network control theory (NCT) is a simple and powerful tool for studying how network topology informs and constrains the dy...
Quantifying genome-wide transcription factor binding affinities for chromatin using BANC-seq
Quantifying genome-wide transcription factor binding affinities for chromatin using BANC-seq
Transcription factors (TFs) bind specific DNA sequences to regulate transcription. Apart from DNA sequences, local factors...
Flux synthesis of two-dimensional covalent organic frameworks
Flux synthesis of two-dimensional covalent organic frameworks
Covalent organic frameworks (COFs) are crystalline porous polymers constructed from organic building blocks into ordered t...
Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Single-cell proteomics by mass spectrometry (MS) allows the quantification of proteins with high specificity and sensitivi...
Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes
Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes
Nanozymes are nanomaterials with enzyme-like catalytic properties. They are attractive reagents because they do not have t...
Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
Complex cellular functions occur via the coordinated formation and dissociation of protein complexes. Functions such as th...
Light-mediated intracellular polymerization
Light-mediated intracellular polymerization
The synthesis of synthetic intracellular polymers offers groundbreaking possibilities in cellular biology and medical rese...
Generating human bone marrow organoids for disease modeling and drug discovery
Generating human bone marrow organoids for disease modeling and drug discovery
The bone marrow supports and regulates hematopoiesis, responding to physiological requirements for blood cell production o...
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
Isotopically carbon-labeled α-amino acids are valuable synthetic targets that are increasingly needed in pharmacology...
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
A promising cell-therapy approach for heart failure aims at differentiating human pluripotent stem cells (hPSCs) into func...
Packaging and containerization of computational methods
Packaging and containerization of computational methods
Methods for analyzing the full complement of a biomolecule type, e.g., proteomics or metabolomics, generate large amounts ...
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins
Single-particle cryo-electron microscopy (cryoEM) provides an attractive avenue for advancing our atomic resolution unders...
Control of DNA replication in vitro using a reversible replication barrier
Control of DNA replication in vitro using a reversible replication barrier
A major obstacle to studying DNA replication is that it involves asynchronous and highly delocalized events. A reversible ...
Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials
Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials
In vivo microscopy of single cells enables following pathological changes in tissues, revealing signaling networks and cel...
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice
Establishing reliable intravenous catheterization in mice with optical implants allows the combination of neural manipulat...
In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation
In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation
The central nervous system (CNS) comprises diverse and morphologically complex cells. To understand the molecular basis of...
Synthesis and functionalization of scalable and versatile 2D protein films via amyloid-like aggregation
Synthesis and functionalization of scalable and versatile 2D protein films via amyloid-like aggregation
Two-dimensional (2D) protein films can be used to modify the properties of surfaces, and find applications predominantly i...
Small RNA structural biochemistry in a post-sequencing era
Small RNA structural biochemistry in a post-sequencing era
High-throughput sequencing has had an enormous impact on small RNA research during the past decade. However, sequencing on...
Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins
Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins
RNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decodin...
Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst
Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst
The tail-to-head terpene (THT) cyclization is a biochemical process that gives rise to many terpene natural product skelet...
A platform for whole-genome speed introgression from Aegilops tauschii to wheat for breeding future crops
A platform for whole-genome speed introgression from Aegilops tauschii to wheat for breeding future crops
Breeding new and sustainable crop cultivars of high yields and desirable traits has been a major challenge for ensuring fo...
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