Gao, X. et al. Establishment of porcine and human expanded potential stem cells. Nat. Cell Biol. 21, 687–699 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wilkinson, A. C. et al. Expanded potential stem cell media as a tool to study human developmental hematopoiesis in vitro. Exp. Hematol. 76, 1–12.e15 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen, A. C. H. et al. Expanded potential stem cells from human embryos have an open chromatin configuration with enhanced trophoblast differentiation ability. Adv. Sci. 10, e2204797 (2023).

Article  Google Scholar 

Ruan, D. et al. Human early syncytiotrophoblasts are highly susceptible to SARS-CoV-2 infection. Cell Rep. Med. 3, 100849 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xu, S., Wang, S., Tam, T., Liu, P. & Ruan, D. Derivation of trophoblast stem cells from human expanded potential stem cells. STAR Protoc. 4, 102354 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ruan, D. et al. An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells. Nat. Protoc. 19, 1710–1749 (2024).

Article  CAS  PubMed  Google Scholar 

Xia, W. et al. Resetting histone modifications during human parental-to-zygotic transition. Science 365, 353–360 (2019).

Article  CAS  PubMed  Google Scholar 

Kumar, B. et al. Polycomb repressive complex 2 shields naive human pluripotent cells from trophectoderm differentiation. Nat. Cell Biol. 24, 845–857 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zijlmans, D. W. et al. Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction. Nat. Cell Biol. 24, 858–871 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang, Q. et al. Adrenomedullin has a pivotal role in trophoblast differentiation: a promising nanotechnology-based therapeutic target for early-onset preeclampsia. Sci. Adv. 9, eadi4777 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen, Y. et al. SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements. Dev. Cell 59, 1506–1522.e11 (2024).

Article  CAS  PubMed  Google Scholar 

Chao, Y. et al. Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis. Signal Transduct. Target Ther. 8, 230 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cao, H. et al. PD-L1 regulates inflammatory programs of macrophages from human pluripotent stem cells. Life Sci. Alliance https://doi.org/10.26508/lsa.202302461 (2024).

Liu, X. et al. Recapitulating primary immunodeficiencies with expanded potential stem cells: proof of concept with STAT1 gain of function. J. Allergy Clin. Immunol. 153, 1125–1139 (2024).

Article  CAS  PubMed  Google Scholar 

Wei, X. et al. A human organoid drug screen identifies alpha2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration. Cell Stem Cell 31, 1813–1830.e8 (2024).

Article  CAS  PubMed  Google Scholar 

Yang, Y. et al. Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell 169, 243–257.e225 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu, B. et al. Chemically defined and xeno-free culture condition for human extended pluripotent stem cells. Nat. Commun. 12, 3017 (2021).

Article  PubMed  PubMed Central  Google Scholar 

Xu, J. et al. Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res. Ther. 10, 193 (2019).

Article  PubMed  PubMed Central  Google Scholar 

Yu, Z. et al. Porcine pluripotent stem cells established from LCDM medium with characteristics differ from human and mouse extended pluripotent stem cells. Stem Cell 40, 751–762 (2022).

Article  Google Scholar 

Zheng, R. et al. Derivation of feeder-free human extended pluripotent stem cells. Stem Cell Rep. 16, 2410–2414 (2021).

Article  Google Scholar 

Guo, G. et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28, 1040–1056 e1046 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yeung, W. S. et al. Frozen–thawed embryo transfer cycles. Hong. Kong Med. J. 15, 420–426 (2009).

PubMed  Google Scholar 

McMahon, A. P. & Bradley, A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell 62, 1073–1085 (1990).

Article  CAS  PubMed  Google Scholar 

Wang, W. et al. Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1. Proc. Natl Acad. Sci. USA 108, 18283–18288 (2011).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Balaban, B. & Gardner, D. K. in Human Gametes and Preimplantation Embryos: Assessment and Diagnosis (eds Gardner, D. K., Sakkas, D., Seli, E. & Wells, D.) 31–43 (Springer, 2013).

Zeng, R. et al. Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2. Stem Cell Res. 40, 101533 (2019).

Article  CAS  PubMed  Google Scholar 

Yan, L. et al. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nat. Struct. Mol. Biol. 20, 1131–1139 (2013).

Article  CAS  PubMed  Google Scholar