Carvalho F, Coonen E, Goossens V, Kokkali G, Rubio C, Meijer-Hoogeveen M, Moutou C, Vermeulen N, De Rycke M. ESHRE PGT Consortium good practice recommendations for the organization of PGT. Hum Reprod Open. 2020;2020(3):hoaa021. https://doi.org/10.1093/hropen/hoaa021.
Article PubMed PubMed Central Google Scholar
Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;344(6268):768–70. https://doi.org/10.1038/344768a0.
Article PubMed CAS Google Scholar
Cimadomo D, Rienzi L, Romanelli V, Alviggi E, Levi-Setti PE, Albani E, Dusi L, Papini L, Livi C, Benini F, Smeraldi A, Patassini C, Ubaldi FM, Capalbo A. Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience. Hum Reprod. 2018;33(10):1839–46. https://doi.org/10.1093/humrep/dey282.
Gosden R. Cryopreservation: a cold look at technology for fertility preservation. Fertil Steril. 2011;96(2):264–8. https://doi.org/10.1016/j.fertnstert.2011.06.029.
Nagy ZP, Shapiro D, Chang CC. Vitrification of the human embryo: a more efficient and safer in vitro fertilization treatment. Fertil Steril. 2020;113(2):241–7. https://doi.org/10.1016/j.fertnstert.2019.12.009.
Article PubMed CAS Google Scholar
Maheshwari A, Raja EA, Bhattacharya S. Obstetric and perinatal outcomes after either fresh or thawed frozen embryo transfer: an analysis of 112,432 singleton pregnancies recorded in the Human Fertilisation and Embryology Authority anonymized dataset. Fertil Steril. 2016;106(7):1703–8. https://doi.org/10.1016/j.fertnstert.2016.08.047.
Taylor TH, Patrick JL, Gitlin SA, Michael Wilson J, Crain JL, Griffin DK. Outcomes of blastocysts biopsied and vitrified once versus those cryopreserved twice foreuploid blastocyst transfer. Reprod Biomed Online. 2014;29(1):59–64. https://doi.org/10.1016/j.rbmo.2014.03.001.
Kato O, Kawasaki N, Bodri D, Kuroda T, Kawachiya S, Kato K, Takehara Y. Neonatal outcome and birth defects in 6623 singletons born following minimal ovarian stimulation and vitrified versus fresh single embryo transfer. Eur J Obstet Gynecol Reprod Biol. 2012;161(1):46–50. https://doi.org/10.1016/j.ejogrb.2011.12.005.
Al Hashimi B, Linara-Demakakou E, Harvey SC, Harvey KE, Griffin DK, Ahuja K, Macklon NS. Double vitrification and warming of blastocysts does not affect pregnancy, miscarriage or live birth rates. Reprod Biomed Online. 2024;49(3): 104103. https://doi.org/10.1016/j.rbmo.2024.104103.
Makieva S, Sachs MK, Xie M, Velasco A, El-Hadad S, Kalaitzopoulos DR, Dedes I, Stiller R, Leeners B. Double vitrification and warming does not compromise the chance of live birth after single unbiopsied blastocyst transfer. Hum Reprod Open. 2023;2023(4):hoad037. https://doi.org/10.1093/hropen/hoad037.
Article PubMed PubMed Central CAS Google Scholar
Zhang Q, Yu W, Jin C, Ni T, Zhou T, Zhao Q, Wang W, Li Y, Yan J. Impact of multiple vitrification-warming procedures and insemination methods on pregnancy and neonatal outcomes in preimplantation genetic testing for aneuploidy. Reprod Sci. 2023;30(7):2302–12. https://doi.org/10.1007/s43032-023-01177-0.
Article PubMed CAS Google Scholar
Theodorou E, Jones BP, Cardenas Armas DF, Heath C, Serhal P, Ben-Nagi J. Live birth rate following a euploid blastocyst transfer is not affected by double vitrification and warming at cleavage or blastocyst stage. J Assist Reprod Genet. 2022;39(4):987–93. https://doi.org/10.1007/s10815-022-02440-0.
Article PubMed PubMed Central Google Scholar
De Vos A, Van Landuyt L, De Rycke M, Verdyck P, Verheyen G, Buysse A, Belva F, Keymolen K, Tournaye H, Verpoest W. Multiple vitrification-warming and biopsy procedures on human embryos: clinical outcome and neonatal follow-up of children. Hum Reprod. 2020;35(11):2488–96. https://doi.org/10.1093/humrep/deaa236.
Van derhoff A, Lanes A, Go K, Dobson L, Ginsburg E, Patel J, Srouji SS. Multiple embryo manipulations in PGT-A cycles may result in inferior clinical outcomes. Reprod Biomed Online. 2024;48(2): 103619. https://doi.org/10.1016/j.rbmo.2023.103619.
Oliva M, Briton-Jones C, Gounko D, Lee JA, Copperman AB, Sekhon L. Factors associated with vitrification-warming survival in 6167 euploid blastocysts. J Assist Reprod Genet. 2021;38(10):2671–8. https://doi.org/10.1007/s10815-021-02284-0.
Article PubMed PubMed Central Google Scholar
Li Piani L, Petrone P, Brutto M, De Vos A, Van Der Kelen A, Vaiarelli A, Rienzi L, Conforti A, Cimadomo D, Verpoest W. A systematic review and meta-analysis of double trophectoderm biopsy and/or cryopreservation in PGT: balancing the need for a diagnosis against the risk of harm. Hum Reprod Update. 2025;31(2):102–15. https://doi.org/10.1093/humupd/dmae031.
Theodorou E, Chronopoulou E, Ozturk O, Brunetti X, Serhal P, Ben-Nagi J. Impact of double trophectoderm biopsy on reproductive outcomes following single euploid blastocyst transfer. Eur J Obstet Gynecol Reprod Biol. 2024;298:35–40. https://doi.org/10.1016/j.ejogrb.2024.04.033.
Bickendorf K, Qi F, Peirce K, Wang R, Natalwala J, Chapple V, Liu Y. Impacts of double biopsy and double vitrification on the clinical outcomes following euploid blastocyst transfer: a systematic review and meta-analysis. Hum Reprod. 2024;39(12):2674–84. https://doi.org/10.1093/humrep/deae235.
Article PubMed PubMed Central Google Scholar
Hao Y, Chen D, Zhang Z, Zhou P, Cao Y, Wei Z, Xu X, Chen B, Zou W, Lv M, Ji D, He X. Successful preimplantation genetic diagnosis by targeted next-generation sequencing on an ion torrent personal genome machine platform. Oncol Lett. 2018;15(4):4296–302. https://doi.org/10.3892/ol.2018.7876.
Article PubMed PubMed Central CAS Google Scholar
Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Reprint of: Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril. 2019;112(4 Suppl1):e81–4. https://doi.org/10.1016/j.fertnstert.2019.08.077.
Luo W, Zheng YM, Hao Y, Zhang Y, Zhou P, Wei Z, Cao Y, Chen D. Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts. BMC Pregnancy Childbirth. 2023;23(1):447. https://doi.org/10.1186/s12884-023-05760-w.
Article PubMed PubMed Central CAS Google Scholar
Schmutzler AG. Theory and practice of preimplantation genetic screening (PGS). Eur J Med Genet. 2019;62(8): 103670. https://doi.org/10.1016/j.ejmg.2019.103670.
Coates A, Kung A, Mounts E, Hesla J, Bankowski B, Barbieri E, Ata B, Cohen J, Munné S. Optimal euploid embryo transfer strategy, fresh versus frozen, after preimplantation genetic screening with next generation sequencing: a randomized controlled trial. Fertil Steril. 2017;107(3):723-7303.e3. https://doi.org/10.1016/j.fertnstert.2016.12.022.
De Vos A, De Munck N. Trophectoderm Biopsy: Present State of the Art. Genes (Basel). 2025;16(2):134. https://doi.org/10.3390/genes16020134.
Article PubMed CAS Google Scholar
Bao S, Yin T, Liu S. Ovarian aging: energy metabolism of oocytes. J Ovarian Res. 2024;17(1):118. https://doi.org/10.1186/s13048-024-01427-y.
Article PubMed PubMed Central Google Scholar
Pasquali R, Patton L, Gambineri A. Obesity and infertility. Curr Opin Endocrinol Diabetes Obes. 2007;14(6):482–7. https://doi.org/10.1097/MED.0b013e3282f1d6cb.
Legge A, Bouzayen R, Hamilton L, Young D. The impact of maternal body mass index on in vitro fertilization outcomes. J Obstet Gynaecol Can. 2014;36(7):613–9. https://doi.org/10.1016/S1701-2163(15)30541-7.
Listorti I, Pirastu G, Ruberti A, Barberi M, Pristerà A, Zazzaro V, Spinella F, Varricchio MT, Viotti M, Greco E, Greco P. Time-lapse analysis of embryos classified as euploid, mosaic, and aneuploid after embryonic trophectoderm biopsy. J Assist Reprod Genet. 2025;42(1):125–38. https://doi.org/10.1007/s10815-024-03364-7.
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