Lipid Visualization of 3D-hetero-type Multicellular Spheroids During the Spatiotemporal Progression of Pancreatic Cancer

Wu H, Fu M, Wu M, Cao Q, Liu Z. Emerging mechanisms and promising approaches in pancreatic cancer metabolism. Cell Death Dis. 2024;15:553.

Article  PubMed  PubMed Central  Google Scholar 

Luo H, Xia X, Huang LB, et al. Pancancer single-cell analysis reveals the heterogeneity and plasticity of cancer-associated fibroblasts in the tumor microenvironment. Nat Commun. 2022;13:6619.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Ho WJ, Jaffee EM, Zheng L. The tumour microenvironment in pancreatic cancer-clinical challenges and opportunities. Nat Rev Clin Oncol. 2020;17:527–40.

Article  PubMed  PubMed Central  Google Scholar 

Unger K, Mehta KY, Kaur P, Wang Y, Menon SS, Jain SK, Moonjelly RA, Suman S, Datta K, Singh R, Fogel P, Cheema AK. Metabolomics based predictive classifier for early detection of pancreatic ductal adenocarcinoma. Oncotarget. 2018;9(33):23078–90.

Article  PubMed  PubMed Central  Google Scholar 

Baba AB, Rah B, Bhat GR, Mushtaq I, Parveen S, Hassan R, Hameed Zargar M, Afroze D. Transforming growth factor-Beta (TGF-β) signaling in cancer-a betrayal within. Front Pharmacol. 2022;13:791272.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Heinrich MA, Mostafa AMRH, Morton JP, Hawinkels LJAC, Prakash J. Translating complexity and heterogeneity of pancreatic tumor: 3D in vitro to in vivo models. Adv Drug Delivery Rev. 2021;174:265–93.

Article  CAS  Google Scholar 

Wu J, Shi Y, Yang S, Tang Z, Li Z, Li Z, Zuo J, Ji W, Niu Y. Current state of stem cell research in non-human primates: an overview. Med Rev. 2023;3:277–304.

Article  Google Scholar 

Han SJ, Kwon S, Kim KS. Challenges of applying multicellular tumor spheroids in preclinical phase. Cancer Cell Int. 2021;21:1–19.

Article  CAS  Google Scholar 

Xie PS, Zhang J, Wu P, Wu Y, Hong Y, Wang J, Cai ZW. Multicellular tumor spheroids bridge the gap between two-dimensional cancer cells and solid tumors: The role of lipid metabolism and distribution. Chin Chem Lett. 2023;34:107349.

Article  CAS  Google Scholar 

Zhang Y, Hu Q, Pei Y, Luo H, Wang Z, Xu X, Zhang Q, Dai J, Wang Q, Fan Z, Fang Y, Ye M, Li B, Chen M, Xue Q, Zheng Q, Zhang S, Huang M, Zhang T, Gu J, Xiong Z. A patient-specific lung cancer assembloid model with heterogeneous tumor microenvironments. Nat Commun. 2024;15:3382.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Lazzari G, Nicolas V, Matsusaki M, Akashi M, Couvreur P, Mura S. Multicellular spheroid based on a triple co-culture: a novel 3D model to mimic pancreatic tumor complexity. Acta Biomater. 2018;78:296–307.

Article  PubMed  CAS  Google Scholar 

Kuen J, Darowski D, Kluge T, Majety M. Pancreatic cancer cell/fibroblast co-culture induces M2 like macrophages that influence therapeutic response in a 3D mode. PLoS ONE. 2017;12(7):e0182039.

Article  PubMed  PubMed Central  Google Scholar 

Zhang A, Qian Y, Ye Z, Chen H, Xie H, Zhou L, Shen Y, Zheng S. Cancer-associated fibroblasts promote M2 polarization of macrophages in pancreatic ductal adenocarcinoma. Cancer Med. 2017;6:463–70.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Shao H, Moller M, Wang D, Ting A, Boulina M, Liu ZJ. A novel stromal fibroblast-modulated 3D tumor spheroid model for studying tumor-stroma interaction and drug discovery. JoVE. 2020;156:e60660.

Google Scholar 

Jiang L, Qin J, Dai Y, et al. Prospective observational study on biomarkers of response in pancreatic ductal adenocarcinoma. Nat Med. 2024;30:749–61.

Article  PubMed  CAS  Google Scholar 

Vogel FCE, Chaves-Filho AB, Schulze A. Lipids as mediators of cancer progression and metastasis. Nat Cancer. 2024;5:16–29.

Article  PubMed  Google Scholar 

Hoffman S, Adeli K. Glucagon-like peptide (GLP)-1 regulation of lipid and lipoprotein metabolism. Med Rev. 2024;4(4):301–11.

Article  Google Scholar 

Zhou X, Xiao Y, Zhou P, Yi SH, Zhao C, Liu JJ. 4D-proteomics-based breast cancer progression. Chin J Anal Lab. 2023;42(4):427–35.

CAS  Google Scholar 

Li Z, Lu Y, Yang L. Imaging and spatial omics of kidney injury: significance, challenges, advances and perspectives. Med Rev. 2023;3(6):514–20.

Article  Google Scholar 

Jiang LX, Yang M, Wali SN, Laskin J. High-throughput mass spectrometry imaging of biological systems: Current approaches and future directions. TrAC-Trends Anal Chem. 2023;163:117055.

Article  CAS  Google Scholar 

Zhao C, Cai ZW. Three-dimensional quantitative mass spectrometry imaging in complex system: from subcellular to whole organism. Mass Spectrom Rev. 2020. https://doi.org/10.1002/mas.21674.

Article  PubMed  Google Scholar 

Chen P, Han Y, Wang L, Zheng Y, Zhu Z, Zhao Y, Zhang M, Chen X, Wang X, Sun C. Spatially resolved metabolomics combined with the 3D tumor-immune cell coculture spheroid highlights metabolic alterations during antitumor immune response. Anal Chem. 2023;95:15153–61.

Article  PubMed  CAS  Google Scholar 

Flint LE, Hamm G, Ready JD, Ling S, Duckett CJ, Cross NA, Cole LM, Smith DP, Goodwin RJA, Clench MR. Characterization of an aggregated three-dimensional cell culture model by multimodal mass spectrometry imaging. Anal Chem. 2020;92:12538–47.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Li L, Zang Q, Li X, Zhu Y, Wen S, He J, Zhang R, Abliz Z. Spatiotemporal pharmacometabolomics based on ambient mass spectrometry imaging to evaluate the metabolism and hepatotoxicity of amiodarone in HepG2 spheroids. J Pharm Anal. 2023;13:483–93.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Ohuchida K, Mizumoto K, Murakami M, Qian LW, Sato N, Nagai E, Matsumoto K, Nakamura T, Tanaka M. Radiation to stromal fibroblasts increases invasiveness of pancreatic cancer cells through tumor-stromal interactions. Cancer Res. 2004;64(9):3215–22.

Article  PubMed  CAS  Google Scholar 

Froeling FEM, Mirza TA, Feakins RM, Seedhar A, Elia G, Hart IR, Kocher HM. Organotypic culture model of pancreatic cancer demonstrates that stromal cells modulate E-cadherin, β-catenin, and ezrin expression in tumor cells. Am J Pathol. 2009;175(2):636–48.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Liu X, Hummon AB. Mass spectrometry imaging of therapeutics from animal models to three-dimensional cell cultures. Anal Chem. 2015;87:9508–19.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Evers TMJ, Holt LJ, Alberti S, Mashaghi A. Reciprocal regulation of cellular mechanics and metabolism. Nat Metab. 2021;3(4):456–68.

Article  PubMed  PubMed Central  Google Scholar 

Faubert B, Solmonson A, DeBerardinis RJ. Metabolic reprogramming and cancer progression. Science. 2020. https://doi.org/10.1126/science.aaw5473.

Article  PubMed  PubMed Central  Google Scholar 

Kattan WE, Liu J, Montufar-Solis D, Liang H, Barathi BB, Hoeven R, Zhou Y, Hancock JF. Components of the phosphatidylserine endoplasmic reticulum to plasma membrane transport mechanism as targets for KRAS inhibition in pancreatic cancer. P Natl Acad Sci. 2021;118(51):e2114126118.

Article  CAS  Google Scholar 

Wolrab D, Jirásko R, Cífková E, et al. Lipidomic profiling of human serum enables detection of pancreatic cancer. Nat Commun. 2022;13(1):124.

Article  PubMed  PubMed Central  CAS  Google Scholar 

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