Integrative single-cell analysis of human colorectal cancer reveals patient stratification with distinct immune evasion mechanisms

The Cancer Genome Atlas Network. Comprehensive molecular characterization of human colon and rectal cancer. Nature 487, 330–337 (2012).

Article  Google Scholar 

Kim, J. C. & Bodmer, W. F. Genomic landscape of colorectal carcinogenesis. J. Cancer Res. Clin. Oncol. 148, 533–545 (2022).

Article  CAS  PubMed  Google Scholar 

La Vecchia, S. & Sebastián, C. Metabolic pathways regulating colorectal cancer initiation and progression. Semin. Cell Dev. Biol. 98, 63–70 (2020).

Article  PubMed  Google Scholar 

Guinney, J. et al. The consensus molecular subtypes of colorectal cancer. Nat. Med. 21, 1350–1356 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dienstmann, R. et al. Consensus molecular subtypes and the evolution of precision medicine in colorectal cancer. Nat. Rev. Cancer 17, 79–92 (2017).

Article  CAS  PubMed  Google Scholar 

Mei, Y. et al. Single-cell analyses reveal suppressive tumor microenvironment of human colorectal cancer. Clin. Transl. Med. 11, e422 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qian, J. et al. A pan-cancer blueprint of the heterogeneous tumor microenvironment revealed by single-cell profiling. Cell Res. 30, 745–762 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang, R. et al. Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors. Genome Med. 14, 93 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Zhang, L. et al. Single-cell analyses inform mechanisms of myeloid-targeted therapies in colon cancer. Cell 181, 442–459.e29 (2020).

Article  CAS  PubMed  Google Scholar 

Pelka, K. et al. Spatially organized multicellular immune hubs in human colorectal cancer. Cell 184, 4734–4752.e20 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee, H.-O. et al. Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer. Nat. Genet. 52, 594–603 (2020).

Article  CAS  PubMed  Google Scholar 

Elmentaite, R. et al. Cells of the human intestinal tract mapped across space and time. Nature 597, 250–255 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

James, K. R. et al. Distinct microbial and immune niches of the human colon. Nat. Immunol. 21, 343–353 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Boland, B. S. et al. Heterogeneity and clonal relationships of adaptive immune cells in ulcerative colitis revealed by single-cell analyses. Sci. Immunol. 5, eabb4432 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Smillie, C. S. et al. Intra- and inter-cellular rewiring of the human colon during ulcerative colitis. Cell 178, 714–730.e220 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li, G. et al. Identification of novel population-specific cell subsets in Chinese ulcerative colitis patients using single-cell RNA sequencing. Cell. Mol. Gastroenterol. Hepatol. 12, 99–117 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mitsialis, V. et al. Single-cell analyses of colon and blood reveal distinct immune cell signatures of ulcerative colitis and Crohn’s disease. Gastroenterology 159, 591–608 (2020).

Article  CAS  PubMed  Google Scholar 

Becker, W. R. et al. Single-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer. Nat. Genet. 54, 985–995 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qi, J. et al. Single-cell and spatial analysis reveal interaction of FAP+ fibroblasts and SPP1+ macrophages in colorectal cancer. Nat. Commun. 13, 1742 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Guo, W. et al. Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing. JCI Insight 7, e152616 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Joanito, I. et al. Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancer. Nat. Genet. 54, 963–975 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xia, J. et al. Single-cell landscape and clinical outcomes of infiltrating B cells in colorectal cancer. Immunology 168, 135–151 (2023).

Article  CAS  PubMed  Google Scholar 

Ozato, Y. et al. Spatial and single-cell transcriptomics decipher the cellular environment containing HLA-G+ cancer cells and SPP1+ macrophages in colorectal cancer. Cell Rep. 42, 111929 (2023).

Article  CAS  PubMed  Google Scholar 

Alshetaiwi, H. et al. Defining the emergence of myeloid-derived suppressor cells in breast cancer using single-cell transcriptomics. Sci. Immunol. 5, eaay6017 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cillo, A. R. et al. Immune landscape of viral- and carcinogen-driven head and neck cancer. Immunity 52, 183–199 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Batlle, E. & Massagué, J. Transforming growth factor-β signaling in immunity and cancer. Immunity 50, 924–940 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ishimoto, T. et al. Activation of transforming growth factor β1 signaling in gastric cancer-associated fibroblasts increases their motility, via expression of rhomboid 5 homolog 2, and ability to induce invasiveness of gastric cancer cells. Gastroenterology 153, 191–204 (2017).

Article  CAS  PubMed  Google Scholar 

Zhan, T., Rindtorff, N. & Boutros, M. Wnt signaling in cancer. Oncogene 36, 1461–1473 (2017).

Article  CAS  PubMed  Google Scholar 

Guo, X. et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nat. Med. 24, 978–985 (2018).

Article  CAS  PubMed  Google Scholar 

Zhang, L. et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature 564, 268–272 (2018).

Article  CAS  PubMed 

Comments (0)

No login
gif