Hetero-Oligomeric Protein Pores for Single-Molecule Sensing

Astier Y, Braha O, Bayley H (2006) Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside 5 ‘-monophosphates by using an engineered protein nanopore equipped with a molecular adapter. J Am Chem Soc 128(5):1705–1710

Article  CAS  PubMed  Google Scholar 

Ayub M, Bayley H (2016) Engineered transmembrane pores. Curr Opin Chem Biol 34:117–126

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ayub M, Stoddart D, Bayley H (2015) Nucleobase recognition by truncated α-hemolysin pores. ACS Nano 9:7895–7903

Article  CAS  PubMed  PubMed Central  Google Scholar 

Baaken G, Halimeh I, Bacri L, Pelta J, Oukhaled A, Behrends JC (2015) High-resolution size-discrimination of single nonionic synthetic polymers with a highly charged biological nanopore. ACS Nano 9(6):6443–6449

Article  CAS  PubMed  Google Scholar 

Banerjee A, Mikhailova E, Cheley S, Gu L-Q, Montoya M, Nagaoka Y, Gouaux E, Bayley H (2010) Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores. Proc Natl Acad Sci 107(18):8165–8170

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bell NA, Engst CR, Ablay M, Divitini G, Ducati C, Liedl T, Keyser UF (2012) DNA origami nanopores. Nano Lett 12(1):512–517

Article  CAS  PubMed  Google Scholar 

Berhanu S, Majumder S, Müntener T, Whitehouse J, Berner C, Bera AK, Kang A, Liang B, Khan N, Sankaran B (2024) Sculpting conducting nanopore size and shape through de novo protein design. Science 385(6706):282–288

Article  CAS  PubMed  Google Scholar 

Boukhet M, Piguet F, Ouldali H, Pastoriza-Gallego M, Pelta J, Oukhaled A (2016) Probing driving forces in aerolysin and α-hemolysin biological nanopores: electrophoresis versus electroosmosis. Nanoscale 8(43):18352–18359

Article  CAS  PubMed  Google Scholar 

Brinkerhoff H, Kang AS, Liu J, Aksimentiev A, Dekker C (2021) Multiple rereads of single proteins at single–amino acid resolution using nanopores. Science 374(6574):1509–1513

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brown CG, Clarke J (2016) Nanopore development at Oxford nanopore. Nat Biotechnol 34(8):810–811

Article  CAS  PubMed  Google Scholar 

Burns JR, Göpfrich K, Wood JW, Thacker VV, Stulz E, Keyser UF, Howorka S (2013) Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor. Angew Chem 52(46):12069

Article  CAS  Google Scholar 

Butler TZ, Pavlenok M, Derrington IM, Niederweis M, Gundlach JH (2008) Single-molecule DNA detection with an engineered MspA protein nanopore. Proc Natl Acad Sci 105(52):20647–20652

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cairns-Gibson DF, Cockroft SL (2022) Functionalised nanopores: chemical and biological modifications. Chem Sci 13(7):1869–1882

Article  CAS  PubMed  Google Scholar 

Cao B, Zhao Y, Kou Y, Ni D, Zhang XC, Huang Y (2014) Structure of the nonameric bacterial amyloid secretion channel. Proc Natl Acad Sci 111(50):E5439–E5444

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cao C, Ying Y-L, Hu Z-L, Liao D-F, Tian H, Long Y-T (2016) Discrimination of oligonucleotides of different lengths with a wild-type aerolysin nanopore. Nat Nanotechnol 11(8):713–718

Article  CAS  PubMed  Google Scholar 

Cardozo N, Zhang K, Doroschak K, Nguyen A, Siddiqui Z, Bogard N, Strauss K, Ceze L, Nivala J (2022) Multiplexed direct detection of barcoded protein reporters on a nanopore array. Nat Biotechnol 40(1):42–46

Article  CAS  PubMed  Google Scholar 

Chen Q, Liu Z (2019) Fabrication and applications of solid-state nanopores. Sensors 19(8):1886

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen M, Khalid S, Sansom MS, Bayley H (2008) Outer membrane protein G: engineering a quiet pore for biosensing. Proc Natl Acad Sci 105(17):6272–6277

Article  CAS  PubMed  PubMed Central  Google Scholar 

Choi L-S, Mach T, Bayley H (2013) Rates and stoichiometries of metal ion probes of cysteine residues within ion channels. Biophys J 105(2):356–364

Article  CAS  PubMed  PubMed Central  Google Scholar 

Clarke J, Wu H-C, Jayasinghe L, Patel A, Reid S, Bayley H (2009) Continuous base identification for single-molecule nanopore DNA sequencing. Nat Nanotechnol 4(4):265–270

Article  CAS  PubMed  Google Scholar 

Crnković A, Srnko M, Anderluh G (2021) Biological nanopores: engineering on demand. Life 11(1):27

Article  PubMed  PubMed Central  Google Scholar 

Derrington IM, Butler TZ, Collins MD, Manrao E, Pavlenok M, Niederweis M, Gundlach JH (2010) Nanopore DNA sequencing with MspA. Proc Natl Acad Sci 107(37):16060–16065

Article  CAS  PubMed  PubMed Central  Google Scholar 

Eifler N, Vetsch M, Gregorini M, Ringler P, Chami M, Philippsen A, Fritz A, Müller SA, Glockshuber R, Engel A (2006) Cytotoxin ClyA from escherichia coli assembles to a 13-meric pore independent of its redox-state. EMBO J 25(11):2652–2661

Article  CAS  PubMed  PubMed Central  Google Scholar 

Faller M, Niederweis M, Schulz GE (2004) The structure of a mycobacterial outer-membrane channel. Science 303(5661):1189–1192

Article  CAS  PubMed  Google Scholar 

Fennouri A, Przybylski C, Pastoriza-Gallego M, Bacri L, Auvray L, Daniel R (2012) Single molecule detection of glycosaminoglycan hyaluronic acid oligosaccharides and depolymerization enzyme activity using a protein nanopore. ACS Nano 6(11):9672–9678

Article  CAS  PubMed  Google Scholar 

Franceschini L, Brouns T, Willems K, Carlon E, Maglia G (2016) DNA translocation through nanopores at physiological ionic strengths requires precise nanoscale engineering. ACS Nano 10(9):8394–8402

Article  CAS  PubMed  PubMed Central  Google Scholar 

Goyal P, Krasteva PV, Van Gerven N, Gubellini F, Van den Broeck I, Troupiotis-Tsaïlaki A, Jonckheere W, Péhau-Arnaudet G, Pinkner JS, Chapman MR (2014) Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG. Nature 516(7530):250–253

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gutsmann T, Heimburg T, Keyser U, Mahendran KR, Winterhalter M (2015) Protein reconstitution into freestanding planar lipid membranes for electrophysiological characterization. Nat Protoc 10(1):188–198

Article  PubMed  Google Scholar 

Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Böhringer N, Schäberle TF, Ranson NA (2023) Darobactin B stabilises a lateral-closed conformation of the BAM complex in E. coli cells. Angew Chem Int Ed 62(34):e202218783

Article  CAS  Google Scholar 

Howorka S (2017) Building membrane nanopores. Nat Nanotechnol 12(7):619–630

Article  CAS 

Comments (0)

No login
gif