Practice Committee of the American Society for Reproductive Medicine. Electronic address: ASRM@asrm.org, Penzias A, Bendikson K, Butts S, Coutifaris C, Falcone T, et al. ASRM standard embryo transfer protocol template: a committee opinion. Fertil Steril 2017;107:897–900. https://doi.org/10.1016/j.fertnstert.2017.02.108.

Practice Committee of the American Society for Reproductive Medicine. Electronic address: ASRM@asrm.org, Practice Committee of the American Society for Reproductive Medicine. Performing the embryo transfer: a guideline. Fertil Steril 2017;107:882–96. https://doi.org/10.1016/j.fertnstert.2017.01.025.

D’Angelo A, Panayotidis C, Alteri A, Mcheik S, Veleva Z. Evidence and consensus on technical aspects of embryo transfer. Hum Reprod Open 2022;2022:hoac038. https://doi.org/10.1093/hropen/hoac038.

Tyler B, Walford H, Tamblyn J, Keay SD, Mavrelos D, Yasmin E, et al. Interventions to optimize embryo transfer in women undergoing assisted conception: a comprehensive systematic review and meta-analyses. Hum Reprod Update. 2022;28:480–500. https://doi.org/10.1093/humupd/dmac009.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Karande VC, Morris R, Chapman C, Rinehart J, Gleicher N. Impact of the “physician factor” on pregnancy rates in a large assisted reproductive technology program: do too many cooks spoil the broth? Fertil Steril. 1999;71:1001–9. https://doi.org/10.1016/s0015-0282(99)00139-9.

Article  PubMed  CAS  Google Scholar 

Hearns-Stokes RM, Miller BT, Scott L, Creuss D, Chakraborty PK, Segars JH. Pregnancy rates after embryo transfer depend on the provider at embryo transfer. Fertil Steril. 2000;74:80–6. https://doi.org/10.1016/s0015-0282(00)00582-3.

Article  PubMed  CAS  Google Scholar 

Angelini A, Brusco GF, Barnocchi N, El-Danasouri I, Pacchiarotti A, Selman HA. Impact of physician performing embryo transfer on pregnancy rates in an assisted reproductive program. J Assist Reprod Genet. 2006;23:329–32. https://doi.org/10.1007/s10815-006-9032-6.

Article  PubMed  Google Scholar 

Yao Z, Vansteelandt S, Van der Elst J, Coetsier T, Dhont M, De Sutter P. The efficacy of the embryo transfer catheter in IVF and ICSI is operator-dependent: a randomized clinical trial. Hum Reprod. 2009;24:880–7. https://doi.org/10.1093/humrep/den453.

Article  PubMed  Google Scholar 

Cirillo F, Patrizio P, Baccini M, Morenghi E, Ronchetti C, Cafaro L, et al. The human factor: does the operator performing the embryo transfer significantly impact the cycle outcome? Hum Reprod. 2020;35:275–82. https://doi.org/10.1093/humrep/dez290.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Cimadomo D, Rienzi L, Conforti A, Forman E, Canosa S, Innocenti F, et al. Opening the black box: why do euploid blastocysts fail to implant? A systematic review and meta-analysis. Hum Reprod Update. 2023;29:570–633. https://doi.org/10.1093/humupd/dmad010.

Article  PubMed  Google Scholar 

ESHRE Clinic PI Working Group, Vlaisavljevic V, Apter S, Capalbo A, D’Angelo A, Gianaroli L, et al. The Maribor consensus: report of an expert meeting on the development of performance indicators for clinical practice in ART. Hum Reprod Open 2021;2021:hoab022. https://doi.org/10.1093/hropen/hoab022.

Fabozzi G, Cimadomo D, Maggiulli R, Vaiarelli A, Ubaldi FM, Rienzi L. Which key performance indicators are most effective in evaluating and managing an in vitro fertilization laboratory? Fertil Steril. 2020;114:9–15. https://doi.org/10.1016/j.fertnstert.2020.04.054.

Article  PubMed  Google Scholar 

Cimadomo D, Capalbo A, Dovere L, Tacconi L, Soscia D, Giancani A, et al. Leave the past behind: women’s reproductive history shows no association with blastocysts’ euploidy and limited association with live birth rates after euploid embryo transfers. Hum Reprod. 2021;36:929–40. https://doi.org/10.1093/humrep/deab014.

Article  PubMed  CAS  Google Scholar 

Vaiarelli A, Cimadomo D, Patrizio P, Venturella R, Orlando G, Soscia D, et al. Biochemical pregnancy loss after frozen embryo transfer seems independent of embryo developmental stage and chromosomal status. Reprod Biomed Online. 2018;37:349–57. https://doi.org/10.1016/j.rbmo.2018.05.019.

Article  PubMed  CAS  Google Scholar 

Maggiulli R, Cimadomo D, Fabozzi G, Papini L, Dovere L, Ubaldi FM, et al. The effect of ICSI-related procedural timings and operators on the outcome. Hum Reprod. 2020;35:32–43. https://doi.org/10.1093/humrep/dez234.

Article  PubMed  Google Scholar 

Capalbo A, Rienzi L, Cimadomo D, Maggiulli R, Elliott T, Wright G, et al. Correlation between standard blastocyst morphology, euploidy and implantation: an observational study in two centers involving 956 screened blastocysts. Hum Reprod. 2014;29:1173–81. https://doi.org/10.1093/humrep/deu033.

Article  PubMed  Google Scholar 

Cimadomo D, Scarica C, Maggiulli R, Orlando G, Soscia D, Albricci L, et al. Continuous embryo culture elicits higher blastulation but similar cumulative delivery rates than sequential: a large prospective study. J Assist Reprod Genet. 2018;35:1329–38. https://doi.org/10.1007/s10815-018-1195-4.

Article  PubMed  PubMed Central  Google Scholar 

Maggiulli R, Giancani A, Cimadomo D, Ubaldi FM, Rienzi L. Human blastocyst biopsy and vitrification. J Vis Exp. 2019. https://doi.org/10.3791/59625.

Article  PubMed  Google Scholar 

Gardner D, Schoolcraft WB. In vitro culture of human blastocyst. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: infertility and genetics beyond. Carnforth: Parthenon Press; 1999. p. 377–88.

Google Scholar 

Cimadomo D, Soscia D, Casciani V, Innocenti F, Trio S, Chiappetta V, et al. How slow is too slow? A comprehensive portrait of day 7 blastocysts and their clinical value standardized through artificial intelligence. Hum Reprod. 2022;37:1134–47. https://doi.org/10.1093/humrep/deac080.

Article  PubMed  Google Scholar 

ESHRE PGT Consortium Steering Committee, Carvalho F, Coonen E, Goossens V, Kokkali G, Rubio C, et al. ESHRE PGT Consortium good practice recommendations for the organisation of PGT. Hum Reprod Open 2020;2020:hoaa021. https://doi.org/10.1093/hropen/hoaa021.

Capalbo A, Treff NR, Cimadomo D, Tao X, Upham K, Ubaldi FM, et al. Comparison of array comparative genomic hybridization and quantitative real-time PCR-based aneuploidy screening of blastocyst biopsies. Eur J Hum Genet. 2015;23:901–6. https://doi.org/10.1038/ejhg.2014.222.

Article  PubMed  CAS  Google Scholar 

Capalbo A, Poli M, Rienzi L, Girardi L, Patassini C, Fabiani M, et al. Mosaic human preimplantation embryos and their developmental potential in a prospective, non-selection clinical trial. Am J Hum Genet. 2021;108:2238–47. https://doi.org/10.1016/j.ajhg.2021.11.002.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Lewin A, Schenker JG, Avrech O, Shapira S, Safran A, Friedler S. The role of uterine straightening by passive bladder distension before embryo transfer in IVF cycles. J Assist Reprod Genet. 1997;14:32–4. https://doi.org/10.1007/BF02765749.

Article  PubMed  PubMed Central  CAS  Google Scholar 

McQueen DB, Robins JC, Yeh C, Zhang JX, Feinberg EC. Embryo transfer training in fellowship: national and institutional data. Fertil Steril. 2020;114:1006–13. https://doi.org/10.1016/j.fertnstert.2020.06.004.

Article  PubMed  Google Scholar 

Miller CM, Weaver AL, Zhao Y, Babayev SN. Outcomes of embryo transfers performed by Reproductive Endocrinology and Infertility fellows vs. faculty: an 11-year retrospective review. Fertil Steril 2022;117:115–22. https://doi.org/10.1016/j.fertnstert.2021.08.044.

Lu MC. Impact of “non-physician factors” on the “physician factor” of in vitro fertilization success: is it the broth, the cooks, or the statistics? Fertil Steril. 1999;71:998–1000. https://doi.org/10.1016/s0015-0282(99)00135-1.

Article  PubMed  CAS  Google Scholar 

Cirillo F, Spadaro D, Morenghi E, Baccini M, Busnelli A, Ronchetti C, et al. Different actors for the same play: the impact of the embryologist performing the embryo transfer. Reprod Biomed Online. 2022;45:661–8. https://doi.org/10.1016/j.rbmo.2022.06.002.

Article  PubMed  Google Scholar 

Glujovsky D, Quinteiro Retamar AM, Alvarez Sedo CR, Ciapponi A, Cornelisse S, Blake D. Cleavage-stage versus blastocyst-stage embryo transfer in assisted reproductive technology. Cochrane Database Syst Rev 2022;5:CD002118. https://doi.org/10.1002/14651858.CD002118.pub6.

Ferreux L, Bourdon M, Sallem A, Santulli P, Barraud-Lange V, Le Foll N, et al. Live birth rate following frozen-thawed blastocyst transfer is higher with blastocysts expanded on day 5 than on day 6. Hum Reprod. 2018;33:390–8. https://doi.org/10.1093/humrep/dey004.

Article  PubMed  Google Scholar 

ESHRE Guideline Group on the Number of Embryos to Transfer, Alteri A, Arroyo G, Baccino G, Craciunas L, De Geyter C, et al. ESHRE guideline: number of embryos to transfer during IVF/ICSI†. Hum Reprod 2024;39:647–57. https://doi.org/10.1093/humrep/deae010.

Practice Committee of the American Society for Reproductive Medicine and the Practice Committee for the Society for Assisted Reproductive Technologies. Electronic address: ASRM@asrm.org. Guidance on the limits to the number of embryos to transfer: a committee opinion. Fertil Steril 2021;116:651–4. https://doi.org/10.1016/j.fertnstert.2021.06.050.

Papageorgiou TC, Hearns-Stokes RM, Leondires MP, Miller BT, Chakraborty P, Cruess D, et al. Training of providers in embryo transfer: what is the minimum number of transfers required for proficiency? Hum Reprod. 2001;16:1415–9. https://doi.org/10.1093/humrep/16.7.1415.

Article  PubMed  CAS  Google Scholar 

Phillips JAS, Martins WP, Nastri CO, Raine-Fenning NJ. Difficult embryo transfers or blood on catheter and assisted reproductive outcomes: a systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2013;168:121–8. https://doi.org/10.1016/j.ejogrb.2012.12.030.

Article  PubMed  Google Scholar 

Zhang HX, Li F, Jin H, Song WY, Su Y, Li G. Effect of retained embryos on pregnancy outcomes of in vitro fertilization: a matched retrospective cohort study. BMC Pregnancy Childbirth. 2023;23:5. https://doi.org/10.1186/s12884-022-05315-5.

Article