Advances in Pharmacology and Pharmacy Vol. 13(3), pp. 390 - 398
DOI: 10.13189/app.2025.130310
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Kalaivani Madhavaram Kuppusamy , Cordelia Mano John , Bhavana Jonnalagadda , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy *
Department of Biomedical Sciences, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai- 6000116. India

ABSTRACT

The current study aimed to understand the effect of Ferulic acid (FA), a phenolic compound with effective antioxidant properties on Tacrolimus (TAC) induced nephrotoxicity in MDCK cell lines. The distal tubular epithelial cell line, MDCK cells were tested for toxicity of TAC and FA using cell viability assay (MTT assay) and the apoptotic and necrotic morphology of cells were studied with AO/EB staining. The cell cycle arrest in TAC and FA co-treated cells was studied using flow cytometry. ROS level was measured using fluorogenic dye, 2,7-dichlorodihydrofluorescein diacetate (DCF-DA) and analysed by flow cytometry. The antioxidant analysis was performed by estimating GSH using 5,5-dithio-bis (2-nitrobenzoic acid) (Ellman's reagent, DTNB) and SOD was measured by the modified NBT method. TAC treated cells showed reduced cell viability, increased apoptosis with cell cycle arrest at S phase and sub G0/G1 phase cell population. But, FA co-treatment significantly (

KEYWORDS
Ferulic Acid, Tacrolimus, Nephrotoxicity, Oxidative Stress, Cell Cycle, Apoptosis

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Kalaivani Madhavaram Kuppusamy , Cordelia Mano John , Bhavana Jonnalagadda , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy , "Attenuation of Tacrolimus Induced Oxidative Stress, Apoptosis and Cell Cycle Arrest by Ferulic Acid in MDCK Cell Lines," Advances in Pharmacology and Pharmacy, Vol. 13, No. 3, pp. 390 - 398, 2025. DOI: 10.13189/app.2025.130310.

(b). APA Format:
Kalaivani Madhavaram Kuppusamy , Cordelia Mano John , Bhavana Jonnalagadda , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy (2025). Attenuation of Tacrolimus Induced Oxidative Stress, Apoptosis and Cell Cycle Arrest by Ferulic Acid in MDCK Cell Lines. Advances in Pharmacology and Pharmacy, 13(3), 390 - 398. DOI: 10.13189/app.2025.130310.