Adapting Mouse Genome Editing Technique from Scratch Using Electroporation

Takahashi G., Gurumurthy C.B., Wada K., Miura H., Sato M., Ohtsuka M. 2015. GONAD: Genome-editing via oviductal nucleic acids delivery system: A novel microinjection independent genome engineering method in mice. Sci. Rep. 5, 11406. https://doi.org/10.1038/srep11406

Article  PubMed  PubMed Central  Google Scholar 

Ohtsuka M., Sato M., Miura H., Takabayashi S., Matsuyama M., Koyano T., Arifin N., Nakamura S., Wada K., Gurumurthy C.B. 2018. I-GONAD: A robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19, 25. https://doi.org/10.1186/s13059-018-1400-x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sato M., Nakamura S., Inada E., Takabayashi S. 2022. Recent advances in the production of genome-edited rats. Int. J. Mol. Sci. 23 (5), 2548. https://doi.org/10.3390/ijms23052548

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hirose M., Tomishima T., Ogura A. 2023. Editing the genome of the golden hamster (Mesocricetus auratus). Methods Mol. Biol. 2637, 247–254. https://doi.org/10.1007/978-1-0716-3016-7_19

Article  CAS  PubMed  Google Scholar 

Namba M., Kobayashi T., Koyano T., Kohno M., Ohtsuka M., Matsuyama M. 2021. GONAD: A new method for germline genome editing in mice and rats. Dev. Growth. Differ. 63 (8), 439–447. https://doi.org/10.1111/dgd.12746

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kobayashi Y., Aoshima T., Ito R., Shinmura R., Ohtsuka M., Akasaka E., Sato M., Takabayashi S. 2020. Modification of i-GONAD suitable for production of genome-edited C57BL/6 inbred mouse strain. Cells. 9 (4), 957. https://doi.org/10.3390/cells9040957

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shang R., Zhang H., Bi P. 2021. Generation of mouse conditional knockout alleles in one step using the i-GONAD method. Gen. Res. 31 (1), 121–130. https://doi.org/10.1101/gr.265439.120

Article  Google Scholar 

Sato M., Nakamura A., Sekiguchi M., Matsuwaki T., Miura H., Gurumurthy C.B., Kakuta S., Ohtsuka M. 2023. Improved genome editing via oviductal nucleic acids delivery (i-GONAD): Protocol steps and additional notes. Methods Mol. Biol. 2631, 325–340. https://doi.org/10.1007/978-1-0716-2990-1_14

Article  CAS  PubMed  Google Scholar 

Melo-Silva C.R., Knudson C.J., Tang L., Kafle S., Springer L.E., Choi J., Snyder C.M., Wang Y., Kim S.V., Sigal L.J. 2023. Multiple and consecutive genome editing using i-GONAD and breeding enrichment facilitates the production of genetically modified mice. Cells. 12 (9), 1343. https://doi.org/10.3390/cells12091343

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gurumurthy C.B., Sato M., Nakamura A., Inui M., Kawano N., Islam M.A., Ogiwara S., Takabayashi S., Matsuyama M., Nakagawa S., Miura H., Ohtsuka M. 2019. Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD. Nat. Protoc. 14 (8), 2452–2482. https://doi.org/10.1038/s41596-019-0187-x

Article  CAS  PubMed  Google Scholar 

Garcia-Frigola C., Carreres M.I., Vegar C., Herrera E. 2007. Gene delivery into mouse retinal ganglion cells by in utero electroporation. BMC Dev. Biol. 7, 103. https://doi.org/10.1186/1471-213X-7-103

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shinmyo Y., Tanaka S., Tsunoda S., Hosomichi K., Tajima A., Kawasaki H. 2016. CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation. Sci. Rep. 6, 20611. https://doi.org/10.1038/srep20611

Article  CAS  PubMed  PubMed Central  Google Scholar 

Book Reviews. 2002. J. Vet. Med. Educ. 29, 245–246. https://doi.org/10.3138/jvme.29.4.245

Article  Google Scholar 

Cagle L.A., Franzi L.M., Epstein S.E., Kass P.H., Last J.A., Kenyon N.J. 2017. Injectable anesthesia for mice: Combined effects of dexmedetomidine, tiletamine-zolazepam, and butorphanol. Anesthesiol. Res. Pract. 2017, 9161040. https://doi.org/10.1155/2017/9161040

Limprasutr V., Sharp P., Jampachaisri K., Pacharinsak C., Durongphongtorn S. 2021. Tiletamine/zolazepam and dexmedetomidine with tramadol provide effective general anesthesia in rats. Animal Model Exp. Med. 4 (1), 40–46. https://doi.org/10.1002/ame2.12143

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cohen J. 2016. “Any idiot can do it.” Genome editor CRISPR could put mutant mice in everyone’s reach. Science. https://doi.org/10.1126/science.aal0334

Modzelewski A.J., Chen S., Willis B.J., Lloyd K.C.K., Wood J.A., He L. 2018. Efficient mouse genome engineering by CRISPR-EZ technology. Nat. Protoc. 13 (6), 1253–1274. https://doi.org/10.1038/nprot.2018.012

Article  CAS  PubMed  PubMed Central  Google Scholar 

Imai Y., Tanave A., Matsuyama M., Koide T. 2022. Efficient genome editing in wild strains of mice using the i-GONAD method. Sci. Rep. 12 (1), 13821. https://doi.org/10.1038/s41598-022-17776-x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Weber E.M., Algers B., Würbel H., Hultgren J., Olsson I.A.S. 2013. Influence of strain and parity on the risk of litter loss in laboratory mice. Reprod. Dom. Anim. 48 (2), 292–296. https://doi.org/10.1111/J.1439-0531.2012.02147.X

Article  CAS  Google Scholar 

Carter D.B., Kennett M.J., Franklin C.L. 2002. Use of perphenazine to control cannibalism in DBA/1 mice. Comp. Med. 52 (5), 452–455. PMID: 12405639

CAS  PubMed  Google Scholar 

Du Sert N.P., Hurst V., Ahluwalia A., Alam S., Avey M.T., Baker M., Browne W.J., Clark A., Cuthill I.C., Dirnagl U., Emerson M., Garner P., Holgate S.T., Howells D.W., Karp N.A., Lazic S.E., Lidster K., MacCallum C.J., Macleod M., Pearl E.J., Petersen O.H., Rawle F., Reynolds P., Rooney K., Sena E.S., Silberberg S.D., Steckler T., Würbel H. 2020. The arrive guidelines 2.0: Updated guidelines for reporting animal research. PLoS Biol. 18 (7), e3000410. https://doi.org/10.1371/journal.pbio.300041

Article  Google Scholar 

Comments (0)

No login
gif