Amendola S, Kneip JS, Meyer F, Perozeni F, Cazzaniga S, Lauersen KJ, Ballottari M, Baier T (2023) Metabolic engineering for efficient ketocarotenoid accumulation in the green microalga Chlamydomonas reinhardtii. ACS Synth Biol 12:820–831

Article  CAS  PubMed  Google Scholar 

Baier T, Kros D, Feiner RC, Lauersen KJ, Muller KM, Kruse O (2018a) Engineered fusion proteins for efficient protein secretion and purification of a human growth factor from the green microalga Chlamydomonas reinhardtii. ACS Synth Biol 7:2547–2557

Article  CAS  PubMed  Google Scholar 

Baier T, Wichmann J, Kruse O, Lauersen KJ (2018b) Intron-containing algal transgenes mediate efficient Recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res 46:6909–6919

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barahimipour R, Strenkert D, Neupert J, Schroda M, Merchant SS, Bock R (2015) Dissecting the contributions of GC content and codon usage to gene expression in the model Alga Chlamydomonas reinhardtii. Plant J 84:704–717

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barolo L, Commault AS, Abbriano RM, Padula MP, Kim M, Kuzhiumparambil U, Ralph PJ, Pernice M (2022) Unassembled cell wall proteins form aggregates in the extracellular space of Chlamydomonas reinhardtii strain UVM4. Appl Microbiol Biotechnol 106:4145–4156

Article  CAS  PubMed  PubMed Central  Google Scholar 

Berndt AJ, Smalley TN, Ren B, Simkovsky R, Badary A, Sproles AE, Fields FJ, Torres-Tiji Y, Heredia V, Mayfield SP (2021) Recombinant production of a functional SARS-CoV-2 Spike receptor binding domain in the green algae Chlamydomonas reinhardtii. PLoS ONE 16:e0257089

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chavez MN, Schenck TL, Hopfner U, Centeno-Cerdas C, Somlai-Schweiger I, Schwarz C, Machens HG, Heikenwalder M, Bono MR, Allende ML, Nickelsen J, Egana JT (2016) Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration. Biomaterials 75:25–36

Article  CAS  PubMed  Google Scholar 

Chen H, Yang QL, Xu JX, Deng X, Zhang YJ, Liu T, Rots MG, Xu GL, Huang KY (2023) Efficient methods for multiple types of precise gene-editing in Chlamydomonas. Plant J 115:846–865

Article  CAS  PubMed  Google Scholar 

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Perez-Perez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trosch R, Westrich LD, Vavitsas K, Baier T, Hubner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD (2018) Birth of a photosynthetic chassis: a MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii. ACS Synth Biol 7:2074–2086

Article  CAS  PubMed  Google Scholar 

Dehghani J, Adibkia K, Movafeghi A, Pourseif MM, Omidi Y (2020) Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2. Bioimpacts 10:259–268

Article  CAS  PubMed  PubMed Central  Google Scholar 

Eichler-Stahlberg A, Weisheit W, Ruecker O, Heitzer M (2009) Strategies to facilitate transgene expression in Chlamydomonas reinhardtii. Planta 229:873–883

Article  CAS  PubMed  Google Scholar 

Einhaus A, Baier T, Kruse O (2024) Molecular design of microalgae as sustainable cell factories. Trends Biotechnol 42:728–738

Article  CAS  PubMed  Google Scholar 

Ferenczi A, Pyott DE, Xipnitou A, Molnar A (2017) Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA. Proc Natl Acad Sci USA 114:13567–13572

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fernandez E, Schnell R, Ranum LP, Hussey SC, Silflow CD, Lefebvre PA (1989) Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 86:6449–6453

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fischer N, Rochaix JD (2001) The flanking regions of PsaD drive efficient gene expression in the nucleus of the green Alga Chlamydomonas reinhardtii. Mol Genet Genomics 265:888–894

Article  CAS  PubMed  Google Scholar 

Freudenberg RA, Baier T, Einhaus A, Wobbe L, Kruse O (2021) High cell density cultivation enables efficient and sustainable Recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresour Technol 323:124542

Article  CAS  PubMed  Google Scholar 

Freudenberg RA, Wittemeier L, Einhaus A, Baier T, Kruse O (2022) Advanced pathway engineering for phototrophic Putrescine production. Plant Biotechnol J 20:1968–1982

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gasser B, Saloheimo M, Rinas U, Dragosits M, Rodríguez-Carmona E, Baumann K, Giuliani M, Parrilli E, Branduardi P, Lang C, Porro D, Ferrer P, Tutino ML, Mattanovich D, Villaverde A (2008) Protein folding and conformational stress in microbial cells producing Recombinant proteins: a host comparative overview. Microb Cell Fact 7:11

Article  PubMed  PubMed Central  Google Scholar 

Gutiérrez S, Overmans S, Wellman GB, Lauersen KJ (2024) Compartmentalized sesquiterpenoid biosynthesis and functionalization in the Chlamydomonas reinhardtii plastid. A European Journal of Chemical Biology, Chembiochem, p e202400902

Google Scholar 

Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, Moroka P, Schroda M, Deponte M (2024) A modular cloning toolkit for the production of Recombinant proteins in leishmania tarentolae. Microb Cell 11:128–142

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jarquin-Cordero M, Chavez MN, Centeno-Cerdas C, Bohne AV, Hopfner U, Machens HG, Egana JT, Nickelsen J (2020) Towards a biotechnological platform for the production of human pro-angiogenic growth factors in the green Alga Chlamydomonas reinhardtii. Appl Microbiol Biotechnol 104:725–739

Article  CAS  PubMed  Google Scholar 

Kiefer AM, Niemeyer J, Probst A, Erkel G, Schroda M (2022) Production and secretion of functional SARS-CoV-2 Spike protein in Chlamydomonas reinhardtii. Front Plant Sci 13:988870

Article  PubMed  PubMed Central  Google Scholar 

Kindle KL (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 87:1228–1232

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kropat J, Hong-Hermesdorf A, Casero D, Ent P, Castruita M, Pellegrini M, Merchant SS, Malasarn D (2011) A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii. Plant J 66:770–780

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lauersen KJ, Berger H, Mussgnug JH, Kruse O (2013) Efficient Recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii. J Biotechnol 167:101–110

Article  CAS  PubMed  Google Scholar 

Lauersen KJ, Huber I, Wichmann J, Baier T, Leiter A, Gaukel V, Kartushin V, Rattenholl A, Steinweg C, von Riesen L, Posten C, Gudermann F, Lutkemeyer D, Mussgnug JH, Kruse O (2015) Investigating the dynamics of Recombinant protein secretion from a microalgal host. J Biotechnol 215:62–71

Article  CAS  PubMed  Google Scholar 

Lopez-Paz C, Liu D, Geng S, Umen JG (2017) Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression. Plant J 92:1232–1244

Article  CAS  PubMed  PubMed Central  Google Scholar 

Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13:e0192433

Article  PubMed  P