In this study, mimetic fluorescence nano-sensor based on molecularly imprinted polymer was designed for detection of amygdalin (AMG) and its characteristic and functional tests were investigated and recorded by ATR-FTIR, AFM and porosity tests. This technique is superior to other reported methods due to the low consumption of materials in its manufacturing and operation as well as its low cost and having desirable performance characteristics such as short response time, high stability and proper detection limit. In-situ surface imprinting was employed by including methacrylic acid as the functional monomer, 2H-chromen-2-one as the fluorescence monomer, AMG as the template molecule, ethylene glycol dimethacrylate as the crosslinking agent, 2,2’-Azobis isobutyronitrile as the initiator and glass slide was used as sensor media. The detection mechanism was based on reducing the intensity of the fluorescence signal due to exposure to a solution containing AMG. The optimum ratio of 1: 6 was obtained for monomer to the cross linking agent and pH = 4.5 as the optimal value. According to the mercury porosity test results more than 91% of the holes created in the surface of the imprinted slides were in the range of 20-66 nm. Linearity analysis was performed by drawing a calibration curve in the range of 0.62-40 µg/mL for the nano fluorosensor and detection limit (LOD) of 0.19 µg/mL and limit of quantification (LOQ) of 0.62 µg/mL were recorded. The response time for the sensor was evaluated in the range of 5-150 sec in buffer solution, urine and serum matrix and 75 sec was recorded as optimal value. In general, the performance characteristics of the designed fluorosensor showed that it has suitable analytical performance for selective detection of AMG in the biological matrix.

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